6023; Takara DNA Ligation Kit, Mighty Mix

Description

This kit is composed of a single solution containing all required ligation reagents (i.e. enzyme and Buffer). All types of ligation can be performed by simply mixing the Ligation Mix and your DNA. This reaction mix can then be directly used for transformations or for in vitro packaging. The Ligation Mix is stable even after repeated freeze-thaw cycles. (*up to 50 cycles). Highly suitable for ligation of blunt-ended DNA and for TA cloning: Good efficiency is obtained not only with sticky-end DNA but also in the cloning of blunt-end DNA or in TA cloning where it is sometimes difficult to get higher efficiency. ·Five-minute ligations are possible: A 5-minute ligation at 25? offers the same efficiency as a 30 min ligation at 16? for vector ligations, thus saving time.