3271; Takara T-Vector PMD™19 (Simple)

Description

T-Vector pMD19 (Simple) is a linearized vector with a single 3’-terminal thymidine at both ends. The T-overhang ends at the cloning site improve the efficiency of ligation of PCR products that contain A-overhangs at 3’-ends. This vector has been deleted the multiple cloning sites in the lac Z gene sequence, but the ß-galactosidase activity is not disrupted. Therefore, the transformant colony having a recombinant plasmid can also be identified by blue/white screening. Due to the lack of multiple cloning sites on the vector, the inserted PCR product cannot be recut using restriction enzymes. When the insert DNA fragment is recut from the vector, the PCR primers should be added to a restriction site to this 5'-end for restriction enzyme digestion and subcloning. T-Vector pMD19 (Simple) is supplied with a Control Insert (500 bp) DNA for positive control reaction.