Premixed Protein Marker (Broad) consists of nine different thermally denatured proteins with molecular weights between 6.5 kDa and 200 kDa. The amount of each protein is normalized to yield uniform band intensities when this marker is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250. This marker contains 0.9 µg of protein per microliter. Five µl of this marker is loaded per lane of an SDS-PAGE mini gel for standard use, providing a sufficient marker for 100 lanes.