The Low Range OG 10 Colored Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Low Range OG 10 Colored Protein Ladder is designed for monitoring protein separated during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, or add a reducing agent before loading.