RR820A; Takara TB Green® Premix Ex Taq™ II (Tli RNase H Plus)

Description

TB Green Premix Ex Taq II (Tli RNaseH Plus) is a reagent specifically designed for intercalator based real-time PCR using TB Green for detection. The premix is supplied as a convenient, ready-to-use 2X concentrate and contains TB Green at a concentration appropriate for real-time monitoring. Simply add primers, template, and sterile purified water to perform intercalator-based real-time PCR. The 2X premix also contains Tli RNaseH, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as the template. This product contains a modified buffer with higher reaction specificity. The inhibition of non-specific amplification, which can interfere with quantification, allows accurate measurement over a wide dynamic range. The combination of this buffer and TaKaRa Ex Taq ® HS, an efficient hot-start PCR enzyme that uses an anti-Taq antibody, allows highly sensitive, reproducible, and reliable real-time PCR amplification and analysis.