TB Green Premix Ex Taq (Tli RNaseH Plus) is designed for intercalator-based real-time PCR using TB Green. It is supplied at a 2X concentration and includes TB Green at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent also contains Tli RNase H, a heat-resistant RNase H that minimizes PCR inhibition by degrading residual mRNA when using cDNA as template. The combination of TaKaRa Ex Taq ® HS, a hot-start PCR enzyme that includes an anti-Taq antibody, and a buffer optimized for real-time PCR, allows high amplification efficiency and detection sensitivity. This product is suitable for high-speed PCR and enables accurate assay and detection of targets, making it possible to obtain highly reproducible and reliable real-time PCR results.