RR086A; Takara One Step TB Green® PrimeScript™ RT-PCR Kit II (Perfect Real Time)

Description

One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) is designed for intercalator-based one-step, real-time RT-PCR using TB Green for detection. All steps of RT-PCR can be performed in a single tube allowing a simple procedure and minimizing the risk of contamination. Also, amplified products are monitored in real time, so there is no need to verify by electrophoresis after PCR. This kit is suitable for detection of tiny amounts of RNA such as RNA virus. This kit uses PrimeScript RTase, which has excellent extendibility and can efficiently synthesize cDNA quickly, and TaKaRa Ex Taq® HS, a high efficiency hot start PCR enzyme, optimized for one step RT-PCR. In comparison to One Step TB Green PrimeScript RT-PCR Kit (Perfect Real Time) (Cat. #RR066A), this kit has an improved buffer system, which results in improved reaction specificity. In addition, this kit achieves simple and convenient procedure with premixed components.