EmeraldAmp GT PCR Master Mix is a 2X premix composed of a DNA polymerase, optimized reaction buffer, dNTPs, and a density reagent. The premix also contains a vivid green dye that will separate into blue and yellow dye fronts when run on an agarose gel. The premix simplifies PCR assembly; simply add primers, template, and water and start the reaction. After PCR, the reaction mixture can be applied directly to a gel for analysis.